DNA Concentration Calculator
This is DNA Concentration Calculator. You can also use it through our mobile application.
The most common purity calculation is the ratio of the absorbance at 260nm divided by the reading at 280nm. Good-quality DNA will have an A260/A280 ratio of 1.7–2.0.
You can calculate the DNA concentration using the formula: concentration [μg/mL] = OD260 * conversion factor.
260/280 ratio of ~1.8 is generally accepted as “pure” for DNA; a ratio of ~2.0 is generally accepted as “pure” for RNA.